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| Test | Specimen Container (Colour) | Sample Type | Comments and special requirements | Reference Ranges (Adult ranges stated unless otherwise specified) | Average turnaround time | Testing Laboratory | Discipline to refer to for clinical advice | |
|---|---|---|---|---|---|---|---|---|
| 17-Alpha-Hydroxyprogesterone | Gel/Serum (Brown) | Blood | Babies should be over 48 hours old before testing | Adult Ranges Male - 0-4.9 nmol/L Female - <5 nmol/L | 3 Weeks | SAS Centre for Steroid | Chemical Pathology | |
| 5-Alpha-Dihydrotestosterone | Gel/Serum (Brown) | Blood | Please refer to report | 4 Weeks | SAS Centre for Steroid | Chemical Pathology | ||
| 5-Hydroxyindole acetic acid (5-HIAA) | 24 Hour Acetic Acid Urine Container | Urine | 0 - 40 mmol/24 hours | 1 Week | Rotherham Hospital Biochemistry | Chemical Pathology | ||
| Acetylcholine Receptor Antibodies | Gel/Serum (Brown) | Blood | 0 - 0.2 nmol/L | 3 Weeks | Sheffield Protein Reference Unit | Chemical Pathology | ||
| Activated Protein C Resistance | Citrate (Citrate) | Blood | 1 x green if requesting APCR only, otherwise refer to Inherited Thrombophilia screen. Part of the thrombophilia screen. Can be requested as an individual test if written in the clinical details. Discuss with Haematology Consultants for testing approval. The citrated bottle must be filled up to the green line marked on the bottle label. Send immediately to the labs for pre analytical processing. The ratio of anticoagulant to blood must be 1:9 therefore under or over-filling the bottle may affect the results and samples may not be tested. | See Referral Report | Sheffield Royal Hallamshire Coagulation | Haematology | ||
| Acquired Thrombophilia Screen (including DRVVT, anti-phospholipid and anti-cardiolipin antibodies) | Citrated and Serum (Green and Brown) | Blood | 3 x Green- Should be received in the laboratory within 4hours of venepuncture. | See Referral Report | 4 weeks | Sheffield Royal Hallamshire Coagulation | Haematology | |
| Acute Kidney Injury score (AKI calculation included as part of an U/E profile) | Gel/Serum (Brown) | Blood | Calculated score of 0-3 indicating possible severity | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Acyl Carnitine Profile | Gel/Serum (Brown) | Blood | Narrative Report | 4 Weeks | Sheffield Children's Hospital | Chemical Pathology | ||
| ADAMTS-13 | Citrate (Green) | Blood | Discuss with Haematology Consultants for testing approval. The citrated bottle must be filled up to the green line marked on the bottle label. The ratio of anticoagulant to blood must be 1:9 therefore under or over-filling the bottle may affect the results and samples may not be tested. Send immediately to the labs for pre analytical processing. Phone the laboratory to inform them that the sample is on its way and is urgent. | See Referral Report | 2 Weeks | Sheffield Royal Hallamshire Coagulation | Haematology | |
| Adenosine Deaminase Assay (ADA) for Diamond-Blackfan Syndrome | EDTA (Red) | Blood | Only requestable by a Consultant Haematologist. | Original report sent straight to the requesting clinician. | 8-12 Weeks | London Purine Research Unit, Guys Hospital | Haematology | |
| Adrenal Antibodies | Gel/Serum (Brown) | Blood | Negative | 4 Weeks | Sheffield Protein Reference Unit | Chemical Pathology | ||
| Adrenocorticotrophic hormone (ACTH) | EDTA (Red) | Blood | Please refer to report | 2 Weeks | Sheffield Royal Hallamshire Hospital | Chemical Pathology | ||
| Alanine Aminotransferase (ALT) | Gel/Serum (Brown) | Blood | See here for information about interference | Adult Range 10 - 49 U/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | |
| Albumin | Gel/Serum (Brown) | Blood | Adult: 35 ? 50 g/L Age 0 to 1 year: 30 - 45g/L 1 year to 16 years: 30 - 50g/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Alcohol | Gel/Serum (Brown) | Blood | Enzymatic assay used - satisfactory for clinical purposes but not for medico-legal purposes. | Mild Intoxication - 50mg/dL Severe Intoxication - >300mg/dL NB. Much inter-individual variation | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | |
| Aldosterone | Lithium Heparin (Orange) | Blood | Following overnight recumbancy - 100 - 450 pmol/L When randomly taken during the day - 100 - 850 pmol/L | 2 Weeks | Leeds SAS Centre | Chemical Pathology | ||
| Alkaline Phosphatase (ALP) | Gel/Serum (Brown) | Blood | Age up to 1 month 70 - 380U/L Age 1 month to 16 years 60 - 425U/L Adult range 30 to 130U/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Alkaline Phosphatase Isoenzymes | Gel/Serum (Brown) | Blood | Available only after discussion with the Chemical Pathologist. | 4 Weeks | Sheffield Royal Hallamshire Hospital | Chemical Pathology | ||
| Alpha thalassaemia DNA analysis | EDTA (Red) | Blood | Only requestable by a Consultant Haematologist. | Refer to the external report. | 8-12 Weeks | Oxford National Haemoglobinopathy Reference Centre | Chemical Pathology | |
| Alpha-1-Acid Glycoprotein (Orosomucoid) | Gel/Serum (Brown) | Blood | Male 5-50 Years- 0.6-1.2 g/L Male over 50 Years- 0.8-2.0 g/L Female 5-50 Years- 0.4-1.0 g/L Female over 50 Years- 0.8-2.0 g/L | 4 Weeks | Sheffield Protein Reference Unit | Chemical Pathology | ||
| Alpha-1-Antitrypsin | Gel/Serum (Brown) | Blood | 1.10 - 2.10 g/L - Adult Range | 4 Weeks | Rotherham Hospital Biochemistry | Chemical Pathology | ||
| Alpha-1-Antitrypsin Phenotype | Gel/Serum (Brown) | Blood | 4 Weeks | Sheffield Protein Reference Unit | Chemical Pathology | |||
| Alpha-2-Macroglobulin | Gel/Serum (Brown) | Blood | Male - 1.3-3.5 g/L (>45yrs of age) Female - 1.4-4.0 g/L (>45yrs of age) | 4 Weeks | Sheffield Protein Reference Unit | Chemical Pathology | ||
| Alpha-Fetoprotein (AFP) | Gel/Serum (Brown) | Blood | 0 - 6.7 kU/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Alpha-Galactosidase ( for Fabry's test) | EDTA | Blood | Narrative Report | Weeks | Manchester, Willink | Chemical Pathology | ||
| Aluminium | Gel/Serum (Brown) | Blood | Or ALU free specimen container. | Please refer to report | 4 Weeks | Sheffield Northern General Hospital | Chemical Pathology | |
| Amino Acids - Plasma | Lithium Heparin (Orange) | Blood | Narrative Report | 3 Weeks | Sheffield Children's Hospital | Chemical Pathology | ||
| Amino Acids - Urine | White Top Universal | Urine | Narrative Report | 3 Weeks | Sheffield Children's Hospital | Chemical Pathology | ||
| Amiodarone | Gel/Serum (Brown) | Blood | 0.6 - 2.5 mg/L | 4 Weeks | Sheffield Royal Hallamshire Hospital | Chemical Pathology | ||
| Ammonia | Lithium Heparin (Orange) | Blood | Sample to be taken in the hospital and delivered by hand promptly to the laboratory ON ICE. Do NOT send via the POD system. | Age up to 1 month <100 umol/L Age above 1 month <40 umol/L | <1 Hour | Barnsley Hospital Biochemistry | Chemical Pathology | |
| Amylase | Gel/Serum (Brown) | Blood | 30 - 118 U/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Amylase - Urine | White Top Universal | Urine | 0 - 650 U/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Amylase Isoenzymes | Gel/Serum (Brown) | Blood | Available only after discussion with the Chemical Pathologist | 4 Weeks | London Great Ormond Street Hospital | Chemical Pathology | ||
| Amyloid | Gel/Serum (Brown) | Blood | Please refer to report | 4 Weeks | Sheffield Protein Reference Unit | Chemical Pathology | ||
| Anabolic Steroids - Urine | 24 Hour Urine (Plain) | Urine | Please refer to report | 4 Weeks | London SAS Steroid Profiling Laboratory | Chemical Pathology | ||
| Anafranil (Clomipramine) | Gel/Serum (Brown) | Blood | 20 - 70 ug/L | 4 Weeks | Sheffield Royal Hallamshire Hospital | Chemical Pathology | ||
| Androstendione | Gel/Serum (Brown) | Blood | Pre-pubertal male and female- <1.4 nmol/L Provisional adult male- 1.3 - 5.8 nmol/L Provisional adult female without PCO in early follicular phase- 1.1 - 5.7 nmol/L | 3 Weeks | Leeds SAS Centre | Chemical Pathology | ||
| Angiotensin converting enzyme (ACE) | Gel/Serum (Brown) | Blood | 20-70 U/L | 1 Week | Royal Hallamshire Hospital | Chemical Pathology | ||
| Ante-natal screening for sickle disease and thalassaemia | EDTA (Red) | Blood | Family Origin questionnaire initiates ante-natal sickle and thalassaemia screening. All information must be completed on the FOQ and signed by the midwife completing the form. FBC must be tested within 24hrs of venepuncture. If not sending straight to lab, store at 4oC. | See haemoglobinopathy screen for reference range. | Tested Monday to Friday TAT 72 Hours | Rotherham Hospital Haematology | Haematology | |
| Anti- Smooth Muscle | Gel/Serum (Brown) | Blood | Sample viable for 10 days | Negative | 2 Weeks | Rotherham Hospital Immunology | Haematology | |
| Anti thrombin III | Citrate (Green) | Blood | Part of the Inherited thrombophilia screen. Can be requested as an individual test if written in the clinical details. 1 x green if requesting APCR only, otherwise refer to Inherited Thrombophilia screen. Discuss with Haematology Consultants for testing approval. The citrated bottle must be filled up to the green line marked on the bottle label. The ratio of anticoagulant to blood must be 1:9 therefore under or over-filling the bottle may affect the results and samples may not be tested. Send immediately to the labs for pre analytical processing (within 4 hours of venepuncture). | Refer to report. | 4 Weeks | Sheffield Royal Hallamshire Coagulation | Haematology | |
| Anti-cardiolipin antibodies (includes IgG & IgM antibodies) | Gel/Serum (Brown) | Blood | Part of the Acquired Thrombophlia screen. Can be requested individually. | IgG - 0-10 GPLIU/ml IgM - 0-7 MPLIU/ml | 4 Weeks | Rotherham Hospital Immunology | Haematology | |
| Anti-CCP antibodies | Gel/Serum (Brown) | Blood | 0 - 5.0 U/mL | <1 Day (Monday to Friday) | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Antidiuretic Hormone (ADH) or Arginine Vasporessin (AVP) | Lithium Heparin (Orange) | Blood | Not routinely available - Discuss with Consultant prior to requesting. | Please refer to report | Newcastle SAS Laboratory | Chemical Pathology | ||
| Anti-gastric parietal cell | Gel/Serum (Brown) | Blood | Sample viable for 10 days. Only tested if specifically requested or if ANA result is positive. | Negative | 2 Weeks | Rotherham Hospital Immunology | Haematology | |
| Anti-Myelin Associated Glycoprotein Antibodies (anti-MAG) | Gel/Serum (Brown) | Blood | Please refer to report | 4 Weeks | Sheffield Protein Reference Unit | Chemical Pathology | ||
| Anti-Neuronal (paraneoplastic) Antibodies | Gel/Serum (Brown) | Blood | Please refer to report | 4 Weeks | Sheffield Protein Reference Unit | Chemical Pathology | ||
| Anti-nuclear antibodies (ANA) | Gel/Serum (Brown) | Blood | Sample viable for 10 days. Positive results reflex for ENA & DNA testing. | Negative. | 2 Weeks | Rotherham Hospital Immunology | Haematology | |
| Anti-nuclear cytoplasmic antibodies (ANCA) | Gel/Serum (Brown) | Blood | Sample viable for 10 days. Positive results are batched and tested weekly for MPO & PR3. | Negative. | 3 Weeks | Rotherham Hospital Immunology | Haematology | |
| Anti-Thyroglobulin | Gel/Serum (Brown) | Blood | Please refer to report | 4 Weeks | Sheffield Protein Reference Unit | Chemical Pathology | ||
| Anti-TNF | Gel/Serum (Brown) | Blood | Refer to external report. | 4 Weeks | City Hospital Birmingham | Chemical Pathology | ||
| Anti-Xa level | Citrate (Green) | Blood | Taken 4 - 6 hours post-injection. The citrated bottle must be filled up to the green line marked on the bottle label. The ratio of anticoagulant to blood must be 1:9 therefore under or over-filling the bottle may affect the results and samples may not be tested. Additional information required, Time and date of last injection, time and date sample was taken, type of heparin. | Refer to external report. | 2 Weeks | Sheffield Royal Hallamshire Coagulation | Haematology | |
| Ascorbic Acid | Gel/Serum (Brown) | Blood | Not routinely available - Discuss with Chemical Pathologist prior to requesting. | Please refer to report | Rotherham Hospital Biochemistry | Chemical Pathology | ||
| Aspartate aminotransferase (AST) | Gel/Serum (Brown) | Blood | Adult range <34 U/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Autoantibody screen: See anti-nuclear antibodies | Gel/Serum (Brown) | Blood | 2 Weeks | Rotherham Hospital Immunology | Haematology | |||
| Basement Membrane Antibodies | Gel/Serum (Brown) | Blood | Negative | 2 Weeks | Rotherham Hospital Biochemistry | Chemical Pathology | ||
| Basophils | EDTA (Red) | Blood | Part of FBC. Refer to FBC for request information. Not requestable alone. | 14D - 0.00-0.20 x109/L 2M - 0.00-0.17 x109/L 1yr - 0.00-0.16 x109/L 12yr - 0.00-0.12 x109/L >12yr - 0.00-0.10 x109/L | Barnsley Hospital Haematology | Haematology | ||
| BCR-ABL (including T315 mutation) | Please see link below for specimen requirements. Http://www.sheffieldchildrens.nhs.uk/SDGS.htm All genetic studies require that patients have been appropriately counselled. Samples collected on Fridays will NOT be processed. | Hardcopy report sent directly to the requesting clinician. | 8-12 Weeks | Sheffield Children's Hospital Genetics Service | Haematology | |||
| Bence Jones Protein - Urine | White Top Universal | Urine | Early AM Urine | Negative | 1 Week | Barnsley Hospital Biochemistry | Chemical Pathology | |
| Beta 2-Microglobulin | Gel/Serum (Brown) | Blood | 1.2 - 2.4 mg/L | 2 Weeks | Rotherham Hospital Biochemistry | Chemical Pathology | ||
| Beta-HCG | Gel/Serum (Brown) | Blood | Pregnancy - >25 U/L Tumour Marker - <5 U/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Bicarbonate | Heparinised Blood Gas Syringe | Venous Gas | Only analysed as part of Blood Gas analysis | 22 - 26 mmol/L | < 1 Hour | Barnsley Hospital Biochemistry | Chemical Pathology | |
| Bile Acids | Gel/Serum (Brown) | Blood | Ideally collect fasting sample (results can be increased in non-fasting samples | <10 umol/L | 1 Week | Rotherham Hospital Biochemistry | Chemical Pathology | |
| Bilirubin - Conjugated | Gel/Serum (Brown) | Blood | 0 - 5 umol/L | <3 Hours | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Bilirubin - Total | Gel/Serum (Brown) | Blood | < 21 umol/L | <3 Hours | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Biotinidase | Lithium Heparin (Orange) | Blood | 2.5 - 10.5 U/L | 4 Weeks | Sheffield Children's Hospital | Chemical Pathology | ||
| Blasts | EDTA (Red) | Blood | Refer to FBC for request information. Not requestable alone. Expressed as an absolute value and percentage of the total white count if seen when examining a blood film. | 0.0-0.00 x109/L | Barnsley Hospital Haematology | Haematology | ||
| Blood film | EDTA (Red) | Blood | EDTA taken from the FBC sample. Can be specifically requested but please provide clinical details. Add ons must be requested asap within 24hrs. Blood film morphology cannot be accurately reviewed after this time. | Blood film generated on basis of FBC results. | Blood films are reviewed within 24hrs. If consultant comments are required TAT can be up to 72hrs. | Barnsley Hospital Haematology | Haematology | |
| Blood Gases | Heparinised Blood Gas Syringe | Arterial Blood | Please notify the laboratory. Please send directly to the laboratory. | pH - 7.35 - 7.45 pCO2 - 4.5 - 6.1 kPa pO2 - 12.0 - 15.0 kPa Bicarbonate - 22 - 26 mmol/L | < 1 Hour | Barnsley Hospital Biochemistry | Chemical Pathology | |
| Bone Alkaline Phosphotase | Gel/Serum (Brown) | Blood | Please refer to report | 3 Weeks | Sheffield Northern General Hospital | Chemical Pathology | ||
| Bone Marrow (Requested via HODS database) | 1 x Bone Marrow Aspirate in EDTA Tube 1 x Bone Marrow Aspirate in Sterile Universal 1 x Bone Marrow Trephine in Specimen Pot with Bone Marrow Medium | Bone Marrow | Only requestable by the Haematologists or after discussion with the Haematology medical team. | See report | Report usually available within 7 days via HODS website. Password access only. | Haematology | ||
| Brain Natriuretic Peptide (BNP) | EDTA (Red) | Blood | Separate EDTA required from FBC/ESR/ HbA1c | BNP <100 ng/L: Heart failure (HF) very improbable BNP 100-500 ng/L: If clinical suspicion of HF then HF probable BNP > 500 ng/L: HF very probable | <3 Hours | Barnsley Hospital Biochemistry | Chemical Pathology | |
| Bromide | Gel/Serum (Brown) | Blood | 0 - 0.25 mmol/L | 4 Weeks | Sheffield Royal Hallamshire Hospital | Chemical Pathology | ||
| C1 Esterase Inhibitor | EDTA (Red) | Blood | Please send 2 x EDTA samples. | 0.15 - 0.43 g/L | 2-3 Weeks | Sheffield Protein Reference Unit | Chemical Pathology | |
| C3 | Gel/Serum (Brown) | Blood | 0.75 - 1.65 g/L | 1 Week | Rotherham Hospital Biochemistry | Chemical Pathology | ||
| C4 | Gel/Serum (Brown) | Blood | 0.14 - 0.54 g/L | 1 Week | Rotherham Hospital Biochemistry | Chemical Pathology | ||
| Caeruloplasmin | Gel/Serum (Brown) | Blood | 0.20 - 0.60 g/L | 1 Week | Rotherham Hospital Biochemistry | Chemical Pathology | ||
| Caffeine | Lithium Heparin (orange) | Blood | Analysed on Tuesday. | On high dose regime 10-35 mg/L | 4 Weeks | Sheffield Children's Hospital | Chemical Pathology | |
| Calcitonin | Gel/Serum (Brown) | Blood | Discuss with Chemical Pathologist before requesting. Fasting serum sample required. Promptly deliver to laboratory on ice. | With report | 2 Weeks | London SAS Laboratories | Chemical Pathology | |
| Calcium - Urine | 24 Hour Urine HCL Urine Container | Urine | 2.5 - 7.5 mmol/24 hours | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Calcium Profile | Gel/Serum (Brown) | Blood | Please see individual tests | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Calcium | Gel/Serum (Brown) | Blood | Reference range for white Caucasions only (may be higher in other ethnic groups) | 1 month 2.0 - 2.7 mmol/L 1 month to 16 years 2.2 - 2.7 mmol/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | |
| Adjusted Calcium | Gel/Serum (Brown) | Blood | Reference range for white Caucasions only (may be higher in other ethnic groups) | Adult Range 2.2 - 2.6 mmol/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | |
| Calprotectin | Blue Top Stool Bottle | Faeces | 2 Weeks | Rotherham Hospital Biochemistry | Chemical Pathology | |||
| Carbamazepine | Gel/Serum (Brown) | Blood | Therapeutic 4 - 12 mg/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Carbohydrate Antigen 125 (CA 125) | Gel/Serum (Brown) | Blood | 0 - 35 kU/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Carbohydrate Antigen 153 (CA 153) | Gel/Serum (Brown) | Blood | 0 - 30 kU/L | 2 Weeks | Sheffield Protein Reference Unit | Chemical Pathology | ||
| Carbohydrate Antigen 199 (CA 199) | Gel/Serum (Brown) | Blood | 0 - 35 kU/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Carbohydrate Deficient Transferrin | Gel/Serum (Brown) | Blood | With report | 4 Weeks | Sheffield Protein Reference Unit | Chemical Pathology | ||
| Carboxyhaemoglobin | Heparinised Blood Gas Syringe | Arterial Blood | Non-smokers = up to 1.5% Smokers = up to 6.5% Heavy smokers = up to 9.0% | When required (Inform the laboratory) | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Carcinoembryonic Antigen (CEA) | Gel/Serum (Brown) | Blood | Non Smokers <2.5 ug/L Smokers <5.0 ug/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Carnitine | Lithium Heparin (Orange) | Blood | Total - 23 - 60 umol/L Free - 15 - 53 umol/L | 4 Weeks | Sheffield Children's Hospital | Chemical Pathology | ||
| Catecholamines - Urine | 24 Hour Urine HCL Container | Urine | This test is subject to interference from a wide variety of drugs and other substances. Please see the table here to find out more | Adrenaline - 0 - 150 nmol/24 hours Noradrenaline - 0 - 570 nmol/24 hours Dopamine - 0 - 3240 nmol/24 hours Metanephrine - 0 - 1.62 æmol/24 hours Normetanephrine - 0 - 2.13 æmol/24 hours | 1 Week | Rotherham Hospital Biochemistry | Chemical Pathology | |
| CD4/CD8 | EDTA (Red) | Blood | Sample must be kept at ambient room temperature. Sample must be tested within 24hrs of venepuncture. Samples should not be sent to the laboratory on Friday. | See report | 4 Weeks | Rotherham Hospital Haematology | Haematology | |
| Cell Markers including T Subsets | EDTA (Red) | Blood | Sample must be kept at ambient room temperature. Sample must be tested within 24hrs of venepuncture. Samples should not be sent to the laboratory on Friday. RHH can only process new suspected acute leukaemia's on Fridays. | Hardcopy report sent to the requesting clinician. | 4 Weeks | Sheffield Royal Hallamshire Hospital, Cell Marker Laboratory | Haematology | |
| Chloride - Serum | Gel/Serum (Brown) | Blood | 95 - 108 mmol/L | < 1 Day - if requested | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Chloride - Urine | 24 Hour Urine Plain Container | Urine | 110 - 250 mmol/24 hours | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Cholesterol (Random) | Gel/Serum (Brown) | Blood | See fasting lipid profile | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Cholinesterase - Acetyl (pesticide exposure) | EDTA (Red) | Blood | Please send 2 x EDTA samples. | 4 Weeks | Buxton Health Sciences Group | Chemical Pathology | ||
| Cholinesterase - Pseudo (scoline sensitivity) | EDTA (Red) | Blood | 4 Weeks | Bristol Cholinesterase Investigation Unit | Chemical Pathology | |||
| Cholinesterase (see Pseudocholinesterase) | Gel/Serum (Brown) | Blood | Details of anaesthetic sensitivity required. | With report | 4 Weeks | Bristol Cholinesterase Investigation Unit | Chemical Pathology | |
| Chromium | EDTA (Red) | Blood | 4 Weeks | Birmingham Trace Elements Laboratory | Chemical Pathology | |||
| Chromogranin A | Gel/Serum (Brown) | Blood | 0 - 60 pmol/L | 3 Weeks | Sheffield Protein Reference Unit | Chemical Pathology | ||
| Chromogranin B | EDTA with Trasylol preservative | Blood | Contact Lab | 4 Weeks | London, SAS Laboratories | Chemical Pathology | ||
| Chromosomes | Patients must have been given genetic counselling prior to any samples being taken. Samples collected on Fridays will not be processed. Please see link below for specimen requirements. Http://www.sheffieldchildrens.nhs.uk/SDGS.htm DNA is stored by SCH after testing. Add on requests should be discussed with SCH directly. Contact details for Sheffield Children's Hospital. Tel:+44(0)11427 17003 (Molecular) Tel:+44(0)11427 17021 (Cyto) | Hardcopy report sent directly to the requesting clinician. | 8-12 Weeks | Sheffield Children's Hospital Genetics Service | Haematology | |||
| Chymotrypsin - Faecal | Blue Top Stool container | Faeces | With report | Referred | Leeds, Department of Clinical Biochemistry | Chemical Pathology | ||
| C-KIT mutation | EDTA (Red) | Peripheral blood or bone marrow in EDTA | Only requestable by Consultant Haematologists. Requests should be booked into HODS by the requesting Clinician. | Refer to HODS database. | 8-12 Weeks | HODS, Sheffield Royal Hallamshire Hospital | Haematology | |
| CKMB | Gel/Serum (Brown) | Blood | 20 - 70 ug/L | Referred | Sheffield Northern General Hospital | Chemical Pathology | ||
| Clomipramine | Gel/Serum (Brown) | Blood | Please refer to report | Referred | Sheffield Royal Hallamshire Hospital | Chemical Pathology | ||
| Coagulation Screen | Citrate (Green) | Blood | Contact the lab for urgent requests. The citrated bottle must be filled up to the green line marked on the bottle label. The ratio of anticoagulant to blood must be 1:9 therefore, under or over-filling the bottle may affect the results and samples may not be tested. Sample viability: 8hrs from venepuncture. Includes :- PT/PTT/FIB | Refer to individual tests for reference ranges | <1 Day | Barnsley Hospital Haematology | Haematology | |
| Conjugated Bilirubin (? Gilberts syndrome) | Gel/Serum (Brown) | Blood | Referred | Sheffield Children's Hospital | Chemical Pathology | |||
| Copper | Gel/Serum (Brown) | Blood | Male - 11-27.2 mmol/L Female Adult <48yrs - 11 - 38.9 mmol/L Female >48yrs - 11-27.2 mmol/L | Rotherham Hospital Biochemistry | Chemical Pathology | |||
| Cortisol - Serum | Gel/Serum (Brown) | Blood | Please note that cortisol should ideally be assessed early in the morning. Adrenal insufficiency can only be excluded by Short Synacthen Test. This method cross-reacts with Hydrocortisone and Prednisolone. For more information on interferences see here | Adult ranges: Morning: 140 - 500 nmol/L Afternoon: 85-460 nmol/L Midnight: <100 nmol/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | |
| Cortisol - Urine | 24 Hour Urine Plain Container | Urine | 10-147 nmol/24 hours | Referred | Leeds SAS Centre for Steroid Hormones | Chemical Pathology | ||
| Cows Milk Antibodies (Lactalbumin, Lactoglobulin & Casein) | Gel/Serum (Brown) | Blood | Please refer to report | Referred | Sheffield Protein Reference Unit | Chemical Pathology | ||
| C-Peptide | Gel/Serum (Brown) | Blood | Separate immediately. | Please refer to report | Referred | Sheffield Royal Hallamshire Hospital or The Clinical Laboratory, Guildford | Chemical Pathology | |
| C-Reactive Protein (CRP) | Gel/Serum (Brown) | Blood | 0 - 5.0 mg/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Creatine Kinase (CK) | Gel/Serum (Brown) | Blood | Up to 3 months 28 - 470 U/L 3 months to 1 year 24 - 240U/L 1 to 11 years 24 - 175U/L 11 to 15 years 24 - 175U/L 15 to 18 years 27 - 145U/L Male above 18 years - 40 - 320 U/L Female above 18 years - 25 - 200 U/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Creatinine (Serum) | Gel/Serum (Brown) | Blood |
For more information on interferences in the creatinine assay please see here | Male, 0 - <14 days: 27 - 81 umol/L Male, 14d - <1yr: 14 - 34 umol/L Male, 1 - <3yr: 15 - 31 umol/L Male, 3 - <5yr: 23 - 37 umol/L Male, 5 - <7yr: 25 - 42 umol/L Male, 7 - <9yr: 30 - 48 umol/L Male, 9 - <11yr: 28 - 57 umol/L Male, 11yr: 36 - 64 umol/L Male, 12yr: 36 - 67 umol/L Male, 13yr: 38 - 76 umol/L Male, 14yr: 40 - 83 umol/L Male, 15yr: 47 - 98 umol/L Male, 16yr: 54 - 99 umol/L Female, 0 - <14 days: 27 - 81 umol/L Female, 14d - <1yr: 14 - 34 umol/L Female, 1 - <3yr: 15 - 31 umol/L Female, 3 - <5yr: 23 - 37 umol/L Female, 5 - <7yr: 25 - 42 umol/L Female, 7 - <9yr: 30 - 48 umol/L Female, 9 - <11yr: 28 - 57 umol/L Female, 11yr: 36 - 64 umol/L Female, 12yr: 36 - 67 umol/L Female, 13yr: 38 - 74 umol/L Female, 14yr: 43 - 75 umol/L Female, 15yr: 44 - 79 umol/L Female, 16yr: 48 - 81 umol/L Adult Male 53 - 97 umol/L Adult Female 44 - 71 umol/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | |
| Creatinine (Urine) | White Top Urine Universal (for random urine) or 24 Hour Urine Plain Container (for 24 hour urine) | Urine | Male 7.1 - 17.7 mmol/24 hours Female 5.3 - 15.9 mmol/24 hours | <1 Day | Chemical Pathology | |||
| Creatinine Clearance | Gel/Serum (Brown) plus 24 Hour Urine Plain container | Brown Top Blood + 24 Hour Plain Urine | 70 - 170 mL/min | < 1 Day | Chemical Pathology | |||
| Cryoglobulins | Contact Laboratory | Blood | Requires sample to be kept warm so it is important that lab is contacted prior to taking samples in order that appropriate arrangements can be made | Narrative Report | As Required | Rotherham Hospital Immunology | Chemical Pathology | |
| CSF - Oligoclonal Bands | CSF Plain Universal plus Gel/Serum (Brown) | CSF in White Top Universal + Brown Blood Sample | Deliver by hand, do NOT use pneumatic tube system. | 4 Weeks | Sheffield Protein Reference Unit | Chemical Pathology | ||
| CSF - Xanthochromia screen | White Top Universal MUST BE PROTECTED FROM LIGHT | CSF | (Min 0.5ml) Final sample required. Sample must be protected from natural light. Sample must be kept in the dark. Deliver by hand, do NOT use pneumatic tube system. | 3 Days | Rotherham Hospital Biochemistry | Chemical Pathology | ||
| CSF -Screen | CSF White Top Universal | CSF | Deliver by hand, do NOT use pneumatic tube system | CSF Protein age dependant: <1 year old 0.1-1.2g/L >1 year old 0.1-0.4g/L CSF glucose should be cross referenced to a serum glucose analysed at the same time. | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Cyclosporin | EDTA (Red) | Blood | Depends on clinical indication | 4 Weeks | Sheffield Northern General Hospital (under normal circumstances) or Sheffield Royal Hallamshire Hospital or Leeds, Department of Transplant Immunology | Chemical Pathology | ||
| Cystine - White Cell/Leucocyte | Lithium Heparin (Orange) | Blood | 4 Weeks | Leeds Department of Chemical Pathology | Chemical Pathology | |||
| Cytogenetics (including basic chromosome analysis, karyotyping and FISH) | Multiple | 1 X Blood in EDTA Container 1 X Blood in Orange Lithium Heparin Container | Refer to external report. | 8-12 Weeks | Chemical Pathology | |||
| Cytokines | Lithium Heparin (Orange) | Blood | Sample must be received by the referral laboratory within 24hours of venepuncture. Samples should not be taken Thursday or Friday. | Refer to external report | 8-12weeks | London St. George?s Hospital | Haematology | |
| D Dimer | Citrate (Green) | Blood | Sample viability: 8hrs from venepuncture. DIC should be added to the request form if this is suspected. Contact the laboratory for urgent requests. The citrated bottle must be filled up to the green line marked on the bottle label. The ratio of anticoagulant to blood must be 1:9 therefore under or over-filling the bottle may affect the results and samples may not be tested. | <0.5 ug/ml | TAT for urgent requests: 45 mins | Barnsley Hospital Haematology | Haematology | |
| Dehydroepiandrosterone Sulphate (DHEAS) | Gel/Serum (Brown) | Blood | Pre adrenarche (1 -5yr) <1 umol/L Female Post Puberty - 24yr = 2.7-11.0 Female 25 - 34yr = 2.1-10.0 Female 35 - 49yr = 1.3-8.5 Female 50 - 59yr = 1.0-7.0 Female 60 - 69yr = <6.0 Female >70yr = <5.0 Male Post Puberty - 24 yr = 3.6-13.0 Male 25 - 34yr = 2.9-12.0 Male 35 - 49yr = 1.7-10.0 Male 50 - 59yr = 1.0-8.0 Male 60 - 69yr = <7.0 Male >70yr = <6.0 | Referred | Leeds SAS Centre for Steroid Hormones | Chemical Pathology | ||
| Digoxin | Gel/Serum (Brown) | Blood | Please ensure samples are collected immediately pre-dose or at least 8 hours post dose. Digoxin toxicity can occur even when serum digoxin concentration is within the therapeutic range; always interpret results in the clinical context. Toxicity can occur at <2.0 ug/L in hypokalaemia, hypoxia, increased sympathetic activity, hypercalcaemia, hypernatraemia, hypomagnesemia, alkalosis or hypothyroidism. Patients treated for digoxin toxicity: Digifab interferes with digoxin immunoassays; no clinical benefit in checking digoxin levels after administration. See: https://cks.nice.org.uk/topics/atrial-fibrillation/prescribing-information/digoxin/ Further information about interference i nthe digoxin assay can be found here | Therapeutic range for Digoxin: 0.7-2.0 ug/L (see NICE Clinical Guideline for Atrial Fibrillation https://cks.nice.org.uk/topics/atrial-fibrillation/prescribing-information/digoxin/. Therapeutic target of 0.5-1.0 ug/L may be appropriate in heart failure Patients treated for digoxin toxicity: Digifab interferes with digoxin immunoassays; no clinical benefit in checking digoxin levels after administration. | Daily | Barnsley Hospital Biochemistry | Chemical Pathology | |
| DNA | Gel/Serum (Brown) | Blood | Sample viable for 10 days. | <20 IU/ml | 1 Week | Rotherham Hospital Immunology | Chemical Pathology | |
| Dopamine - Urine | 24 Hour Urine HCl Container | Urine | 2 Weeks | Rotherham Hospital Biochemistry | Chemical Pathology | |||
| Downs Screening (Double Test) | Gel/Serum (Brown) | Blood | Request to be ordered only on specific Downs Screening stationary. | Sheffield Department of Immunology | Contact Immunology directly. | |||
| Drugs of Abuse - Confirmation & Identification of Opiates - Urine | White Top Universal | Urine | Referred only when specifically requested following positive identification of opiates within urine drug screen. | 4 Weeks | City Hospital Birmingham, Trace Elements laboratory | Chemical Pathology | ||
| Drugs of Abuse Screen - Urine | White Top Universal | Urine | Please note that this screening method does not accurately identify the presence of MDMA and MDA | Negative Positive | <1 Week | Chemical Pathology | ||
| Elastase | Blue Top Universal | Faeces | 2 Weeks | Sheffield Royal Hallamshire Hospital | Chemical Pathology | |||
| Electrophoresis - Protein | Gel/Serum (Brown) | Blood | Narrative Report | 1 Week | Rotherham Hospital Immunology | Chemical Pathology | ||
| EMA screen (for hereditary spherocytosis) | EDTA (Red) | Blood | Minimum of 0.5ml of sample (Sample must be <48 hours old). Testing should be performed on patients >1year old as results may not be interpretable. | Refer to report. | 2 Weeks | Sheffield Children's Hospital Haematology | Haematology | |
| ENA Screen | Gel/Serum (Brown) | Blood | Sample viable for 10 days. | Negative Positive screens have further confirmatory testing performed. | 2 Weeks | Rotherham Hospital Immunology | Haematology | |
| Endomysial Antibodies (IgA) | Gel/Serum (Brown) | Blood | Negative | 2 Weeks | Rotherham Hospital Immunology | Chemical Pathology | ||
| Endomysial Antibodies (IgG) | Gel/Serum (Brown) | Blood | Usually laboratory initiated test. | Negative | 3 Weeks | Rotherham Hospital Immunology | Chemical Pathology | |
| Eosinophils | EDTA (Red) | Blood | Part of the FBC. Refer to FBC for request information. | 3D = 0.05 - 1.23 x109/L 14D = 0.05 - 1.00 x109/L 10yr = 0.05 - 0.88 x109/L 11yr = 0.05 - 0.80 x109/L 15yr = 0.05 - 0.70 x109/L 16yr = 0.05 - 0.60 x109/L >16yr = 0.05 - 0.45 x109/L | <1 Day | Barnsley Hospital Haematology | Haematology | |
| Erythrocyte Sedimentaiton Rate (ESR) | EDTA (Red) | Blood | 1ml minimum required for ESR testing. Sample viability 24 hours. Lab must be informed of urgent requests for temporal arteritis. | <70yrs = 1-15 mm/hr >70yrs: 1-30 mm/hr | 1 Hour (Urgent tests), 4 Hours (Routine tests) | Barnsley Hospital Haematology | Haematology | |
| Erythropoietin Levels (Serum) | Gel/Serum (Brown) | Blood | Sample viable for 10 days. For inofrmation on interferences in the erythropoietin assay, please see here | 3.0-18.0 mIU/ml | 6 Weeks | Rotherham Hospital Haematology | Haematology | |
| Ethosuximide | Gel/Serum (Brown) | Blood | 4 Weeks | Sheffield Royal Hallamshire Hospital | Chemical Pathology | |||
| Ethylene Glycol | Gel/Serum (Brown) | Blood | <2 Days | Sheffield Northern General Hospital, Toxicology Department | Chemical Pathology | |||
| Exon 12 mutation | EDTA (Red) | Blood | Only requestable by the Haematology Consultants. Must be requested via the HODS database by the requesting clinician. | Refer to HODS database | 6 Weeks | HODS, Sheffield Royal Hallamshire Hospital | Haematology | |
| Fabry's (alpha - galactosidase) | Blood Spot | Blood Spot | Narrative Report | 4 Weeks | Manchester, Willink | Chemical Pathology | ||
| Factor Assays | Citrate (Green) | Blood | 2-4 green (adult or paediatric), depending on how many factors are requested. Send to the laboratory immediately. Requests for factor assays should be approved by the Consultant Haematologists. Please discuss with them before sending a request, otherwise testing may be delayed. Urgent requests should be telephoned to the lab | See individual factors for reference ranges. | TAT is dependent upon the urgency of the request. Routine requests may take up to two weeks for a report to be generated. | Sheffield Royal Hallamshire Hospital, Coagulation | Haematology | |
| Factor II | Citrate (Green) | Blood | Refer to factor assays test | 1D = 0.41-0.69 Units/L 3D = 0.50-0.73 Units/L 1yr = 0.62-1.03 Units/L 5yr = 0.70-1.09 Units/L 10yr = 0.67-1.10 Units/L 16yr = 0.61-1.07 Units/L >18yr = 0.78-1.38 Units/L | See Factor assays | See Factor assays | Haematology | |
| Factor IX | Citrate (Green) | Blood | Refer to factor assays test | 1D = 0.35-0.56 Units/L 3D = 0.44-0.97 Units/L 1yr = 0.43-1.21 Units/L 5yr = 0.44-1.27 Units/L 10yr = 0.48-1.45 Units/L 16yr = 0.64-2.16 Units/L >18yr = 0.59-2.54 Units/L | See Factor assays | See Factor assays | Haematology | |
| Factor V | Citrate (Green) | Blood | Refer to factor assays test | 1D = 0.64-1.03 Units/L 3D = 0.92 - 1.54 Units/L 1yr = 0.94 - 1.41 Units/L 5yr = 0.67 - 1.27 Units/L 10yr = 0.56 - 1.41 Units/L 16yr = 0.67 - 1.41 Units/L >18yr = 0.78-1.52 Units/L | See Factor assays | See Factor assays | Haematology | |
| Factor V Leiden (Genetics) See Inherited Thrombophilia testing if requesting as part of a battery. | EDTA (Red) | Blood | Send immediately to the laboratory. All requests are audited to ensure that these tests are performed only where appropriate. The criteria for testing are based on BCSHGuideline: Investigation & Management of Heritable Thrombophilia BJH 114:512-528 2001. Requests without clinical details or with inadequate details will not be processed. The samples are saved for 3 months. Please contact Haematology clinicians if the request has been denied and there are other clinical indicators for testing. Is requestable as an individual test but will only be performed if the Activated Protein C Resistance result is abnormal. | Refer to external report. | 4 Weeks | Haematology | ||
| Factor VII | Citrate (Green) | Blood | Refer to factor assays test | 1D = 0.52-0.88 Units/L 3D = 0.67-1.07 Units/L 1yr = 0.83-1.60 Units/L 5yr = 0.72-1.50 Units/L 10yr = 0.70-1.56 Units/L 16yr = 0.69-2.00 Units/L | See Factor assays | See Factor assays | Haematology | |
| Factor VIII | Citrate (Green) | Blood | Refer to factor assays test | 1D = 1.05-3.29 Units/L 3D = 0.83-2.74 Units/L 1yr = 0.54-1.45 Units/L 5yr = 0.36-1.85 Units/L 10yr = 0.52-1.82 Units/L 16yr = 0.59-2.00 Units/L >18yr = 0.52-2.90 Units/L | See Factor assays | See Factor assays | Haematology | |
| Factor X | Citrate (Green) | Blood | Refer to factor assays test | 1D = 0.46-0.67 Units/L 3D = 0.46-0.75 Units/L 1M = 0.77-1.22 Units/L 5M = 0.72-1.25 Units/L 10M = 0.68-1.25 Units/L 16yr = 0.53-1.22 Units/L >18yr = 0.96-1.71 Units/L | See Factor assays | See Factor assays | Haematology | |
| Factor XI | Citrate (Green) | Blood | Refer to factor assays test | 1D = 0.07-0.41 Units/L 3D = 0.24-0.79 Units/L 1yr = 0.62-1.25 Units/L 5yr = 0.65-1.62 Units/L 10yr = 0.65-1.62 Units/L 16yr = 0.65-1.30 Units/L >18yr = 0.67-1.96 Units/L | See Factor assays | See Factor assays | Haematology | |
| Factor XII | Citrate (Green) | Blood | Refer to factor assays test | 1D = 0.43-0.80 Units/L 3D = 0.13-0.97 Units/L 1yr = 0.20-1.35 Units/L 5yr = 0.36-1.35 Units/L 10yr = 0.26-1.37 Units/L 16yr = 0.14-1.17 Units/L >18yr = 0.35-2.07 Units/L | See Factor assays | See Factor assays | Haematology | |
| Factor XIII | Citrate (Green) | Blood | Refer to factor assays test | See Factor assays | See Factor assays | See Factor assays | Haematology | |
| Faecal Calprotectin | Blue top Stool Bottle | Faeces | 2 Weeks | Rotherham Hospital Biochemistry | Chemical Pathology | |||
| Faecal Elastase see Elastase | Blue Top Universal | Faeces | 2 Weeks | Sheffield Royal Hallamshire Hospital | ||||
| FBC Includes: Platelets, White cell count, Red count, MCV, MCH, MCHC, Hct, RDW, LUC and White cell differential (neutrophils, lymphocytes, monocytes, eosinophils & basophils) | EDTA (Red) | Blood | Minimum 50ul. The laboratory must be phoned/bleeped for urgent requests. | For reference ranges, refer to individual tests. | Routine <2 hours Urgent <1 hour | Barnsley Hospital Haematology | Haematology | |
| Ferritin (Serum) | Gel/Serum (Brown) | Blood | Consider measuring blood lead in any child presenting with possible iron deficiency and pica. | Male 22 - 322 ug/L Female 10 - 291 ug/L | <1 day | Barnsley Hospital Haematology | Haematology | |
| Fibrinogen | Citrate (Green) | Blood | Part of Coagulation Screen. Refer to Coagulation Screen | 1 day = 1.92-3.74 g/l 3 days = 2.83-4.01 g/l 1 yr = 0.82-3.83 g/l >16yrs = 1.90-4.10 g/l | Barnsley Hospital Haematology | Haematology | ||
| Fish Odour Syndrome (Trimethylaminuria) | 24 Hour Acid HCL Urine Container | Urine | Sheffield Children's Hospital | Chemical Pathology | ||||
| Flecainide | Gel/Serum (Brown) | Blood | 200 - 800 ug/L | 4 Weeks | Sheffield Royal Hallamshire Hospital | Chemical Pathology | ||
| Folate (Serum) | Gel/Serum (Brown) | Blood | 1 x 4.9ml | >5.4 ug/L | <1 day | Barnsley Hospital Haematology | Haematology | |
| Follicle Stimulating Hormone (FSH) | Gel/Serum (Brown) | Blood | Follicular = 2.5 - 10.2 U/L Mid - Cycle = 3.4 - 33.4 U/L Luteal = 1.5 - 9.1 U/L Post menopausal = 23.0 - 116.3 U/L Male = 1.4 - 18.1 U/L | <1 day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Free Androgen Index (Calculated from testosterone and SHBG) | Gel/Serum (Brown) | Blood | Adult male <50 years = 14.5-80.3 Adult male >50 years = 9.4-52.5 Female pre menopausal = 0.3-9.8 Female post menopausal = 0.2-5.9 | Barnsley Hospital Biochemistry | Chemical Pathology | |||
| Free Fatty Acids | Fluoride | Blood | See Hypoglycemia Screen | Barnsley Hospital Biochemistry | Chemical Pathology | |||
| Free Light Chains (serum) | Gel/Serum (Brown) | Blood | Free Kappa = 3.3 - 19.4 mg/L Free Lambda = 5.7 - 26.3 mg/L Kappa/Lambda ration = 0.26 - 1.65 | 3 Weeks | Sheffield Protein Reference Unit | Chemical Pathology | ||
| Free Light Chains (urine) | White Top Universal | Urine | 3 Weeks | Sheffield Protein Reference Unit | Chemical Pathology | |||
| Free Thyroxine (fT4) | Gel/Serum (Brown) | Blood | Adult range = 10.0 - 18.7 pmol/L Please contact laboratory if age related ranges required | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Free Tri-iodothyronine (fT3) | Gel/Serum (Brown) | Blood | Adult range = 3.5 - 6.5 pmol/L Please contact laboratory if age related ranges required | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Fructosamine | Gel/Serum (Brown) | Blood | 4 Weeks | Middlesex, Department of Clinical Biochemistry | Chemical Pathology | |||
| Galactosaemia Screen | Lithium Heparin (Orange) | Blood | Negative | 3 Weeks | Sheffield Children's Hospital | Chemical Pathology | ||
| Gamma-Glutamyl Transferase | Gel/Serum (Brown) | Blood | Adult Male - 0-73 U/L Adult Female - 0-38 U/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Gastrin | EDTA Contact Laboratory in advance | Please contact the laboratory (01226) 432733. Must be delivered within 15 minutes of the draw | Part of gut hormone screen. Fasting sample required. Stop PPls 2 weeks before and H2 blockers 3 days before sampling, for more details contact Duty Biochemist. | 0 - 40 pmol/L | 4 Weeks | London, SAS Laboratories | Chemical Pathology | |
| Gene studies | EDTA (Red) | For Sample requirements visit: http://www.sheffieldchildrens.nhs.uk/sdgs.htm All genetic studies require patients to have been appropriately counselled. GPs requesting these studies should contact the referral lab directly to obtain approval and specimen requirements before proceeding with sample collection. Contact details for Sheffield Children's Hospital:Tel:+44(0)11427 17003 (Molecular) Samples collected on Fridays will not be processed. DNA is stored by SCH after testing. Add on requests should be discussed with SCH directly. | Hardcopy report sent directly to the requesting clinician from the referral laboratory. | 8-12 Weeks | Sheffield Children's Hospital Genetics Service | Haematology | ||
| Genetics | EDTA (Red) | See Gene Studies | Referred | |||||
| Gentamicin | Gel/Serum (Brown) | Blood | If advice is required please contact pharmacy | <1 Day | Barnsley Hospital Biochemistry | |||
| Glandular Fever Screen | Gel/Serum (Brown) | Blood | Sample viable for 24 hours or 48 hours if kept between 2-8C. Should be requested with an FBC. | Negative | <1 Day | Barnsley Hospital Haematology | Haematology | |
| Gliadin Antibodies | Gel/Serum (Brown) | Blood | Negative | 2 Weeks | Rotherham Hospital Immunology | Chemical Pathology | ||
| Glomerular Basement Membrane Abs (GBM) | Gel/Serum (Brown) | Blood | 0 - 20 U/mL | 3 Weeks | Rotherham Hospital Immunology | Haematology | ||
| Glucagon | Contact Laboratory | BD Gastrin Vacutainer | Contact Laboratory for container | 0 - 50 pmol/L (Part of gut hormone screen) | Referred | London SAS Centre | Chemical Pathology | |
| Glucose | Gel/Serum (Brown) | Blood | Normal fasting = < 7.0 mmol/L Fasting indicative of Diabetes Mellitus = > 7.0 mmol/L Random indicative of Diabetes Mellitus = > 11.1 mmol/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Glucose-6-Dehydrogenase Phosphate (G6PD) | EDTA (Red) | Blood | Minimum 50ul. Testing will only be carried out in accordance with the following guidelines: A possible drug induced haemolytic episode is suspected with testing limited to those following use of Glibenclamide, Nalidixic Acid, Nitrofurantoin and Primaquine. A positive family history In neonates who have definite evidence of a congenital non-spherocytic haemolytic anaemia. A patient is being screened for G6PD prior to being started on KRYSTEXXA therapy for gout NB reticulocytosis will cause an increase in G6PD levels negating the usefulness of testing in this setting. | Refer to the external report | Referred - 2 Weeks | Sheffield Royal Hallamshire Hospital, Haemolysis Laboratory | Haematology | |
| Glycated Haemoglobin (HbA1c) | EDTA (Red) | Blood | In diabetes aim for <53 mmol/mol In adults, HbA1c of greater or equal to 48 mmol/mol is the recommended cut-off for diagnosing diabetes mellitus (repeat measurement required in asymptomatic individuals).See this page for further information on use of HbA1c, including limitations | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Growth Hormone | Gel/Serum (Brown) | Blood | Adult Male = 0-2.5 ug/L Adult Female = 0.13-9.9 ug/L | 3 Weeks | Sheffield Royal Hallamshire Hospital | Chemical Pathology | ||
| Gut Hormones | EDTA Contact lab to arrange in advance | Please contact the laboratory (01226) 432733. Must be delivered within 15 minutes of the draw | Please refer to information for Gastrin. | Gastrin = <40 pmol/L Glucagon = <50 pmol/L Neurotensin (specific request) = <100 pmol/L Pancreatic Poypeptide = <300 pmol/L Somatostatin = <150 pmol/L VIP = <30 pmol/L Chromogranin A = <60 pmol/L Chromogranin B = <150 pmol/L | Referred | London, SAS Laboratories | Chemical Pathology | |
| Haemochromatosis gene testing (HFE gene) | EDTA (Red) | Blood | 2 x EDTA samples required. Routine screening should be carried out prior to genetic testing. Requests are only approved if appropriate clinical details and previous blood results are indicative for genetic testing. Ideally should not be taken on Fridays. | Refer to the external report | Referred 8-12 weeks | Sheffield Diagnostic Genetic Service, Sheffield Children's Hospital | Chemical Pathology | |
| Haemoglobin | EDTA (Red) | Blood | Part of the FBC. Not requestable alone. | 3D = 145-230 7D = 140-222 14D = 130-195 30D = 115-167 2M = 94-132 3M = 98-132 4M = 102-137 6M = 103-137 1YR = 105-137 2YR = 107-134 3YR = 110-135 5YR = 110-136 6YR = 111-138 7YR = 113-142 8YR = 115-142 10YR = 117-145 11YR = 118-145 12YR = 120-147 Male 14YR = 124-156 Male 16YR = 131-163 Male 18YR = 129-165 Male >=21YR = 132-169 Female 14YR = 132-169 Female 16YR = 117-156 Female 18YR = 117-157 Female >=21YR = 119-149 | Barnsley Hospital Haematology | Haematology | ||
| Haemoglobin A2 (HbA2) | EDTA (Red) | Blood | Part of the Haemoglobinopathy screen. Not requestable alone. | 2.0-3.5% | Barnsley Hospital Haematology | Haematology | ||
| Haemoglobin F (HbF) | EDTA (Red) | Blood | Part of the Haemoglobinopathy screen. Not requestable alone. | <2 % HbF<4.0% in pregnancy | Barnsley Hospital Haematology | Haematology | ||
| Haemoglobin Variants | EDTA (Red) | Blood | Refer to Haemoglobinopathy screen. | Negative | Haematology | |||
| Haemoglobinopathy screen Includes Haemoglobin A2, Haemoglobin F, FBC, Blood Film, Ferritin | Tested Mon-Fri For urgent sickle cell screening for pre-operative cases or ?Sickle cell crises refer to sickle screen. For Partner testing, please provide the details of woman. For antenatal screening, refer to FOQ testing. | Refer to individual tests listed in the haemoglobinopathy screen for reference ranges | TAT for full HPLC report 72hrs | Rotherham Hospital Haematology Positive results/variants are referred to Nottingham Queen?s Medical Centre for confirmation. | Haematology | |||
| Haptoglobin | Gel/Serum (Brown) | Blood | 0.4 - 2.8 g/L | 1 Week | Rotherham Hospital Biochemistry | Chemical Pathology | ||
| HCT (Haematocrit) | EDTA (Red) | Blood | Part of the FBC. Not requestable alone. | 7D = 0.44-0.70 L/L 14D = 13.0-19.5 L/L 30D = 0.34-0.55 L/L 2M = 0.29-0.47 L/L 3M = 0.29-0.41 L/L 6M = 0.30-0.41 L/L 1YR = 0.31-0.41 L/L 3YR = 0.32-0.41 L/L 5YR = 0.33-0.41 L/L 7YR = 0.33-0.41 L/L 8YR = 0.34-0.44 L/L Male 14YR = 0.35-0.47 L/L Male 16YR = 0.37-0.49 L/L Male >=18YR = 0.39-0.49 L/L Female 14YR = 0.35-0.45 L/L Female 16YR = 0.35-0.45 L/L Female >=18YR = 0.35-0.46 L/L | Barnsley Hospital Haematology | Haematology | ||
| Heparin Induced Thrombocytopenia (HIT) | Citrate (Green) | Blood | RHH specific request information must be completed. Type of heparin, dosage and date & time of last dose should be included. RHH HIT request information | Refer to external report | 2 Weeks | Sheffield Royal Hallamshire Hospital, Coagulation | Haematology | |
| Homocysteine | EDTA (Red) | Blood | Separate within 1 hour Fasting sample required Deliver immediately to the laboratory - separate within 1 hour | Male = 0 - 18 umol/L Female = 0 - 16 umol/L | 1 Week | Sheffield Children's Hospital | Chemical Pathology | |
| IFA (Intrinsic Factor Antibodies) | Gel/Serum (Brown) | Blood | Negative/Positive | Samples processed < 1 Day. Positive screens have further confirmatory testing performed. TAT: 1wk | Rotherham Hospital Immunology | Immunology | ||
| IgE - total | Gel/Serum (Brown) | Blood | Allergen specific IgE antibodies can be found without clinical reactions, especially in atopic individuals. They are NOT proof of allergy and are not useful for screening. Suggest referral to the allergy clinic at NGH, Sheffield if indicated | 0 - 81kU/L Grade 0 = 0.0 - 0.35 kU/L Negative Grade 1 = 0.35 - 0.7 kU/L Weak Positive Grade 2 = 0.7 - 3.5 kU/L Positive Grade 3 = 3.5 - 17.5 kU/L Positive Grade 4 = 17.5 - 50.0 kU/L Strong Positive Grade 5 = 50.0 - 100 kU/L Strong Positive Grade 6 = >100 kU/L Strong Positive | 2 Weeks | Rotherham Hospital Immunology | Chemical Pathology | |
| IgG Subclasses | Gel/Serum (Brown) | Blood | IgG1 = 3.2 - 10.2 g/L IgG2 = 1.2 - 6.6 g/L IgG3 = 0.2 - 1.9 g/L | 3 Weeks | Sheffield Protein Reference Unit | Chemical Pathology | ||
| Immunofixation | Gel/Serum (Brown) | Blood | Narrative Report | As Required | Rotherham Hospital Immunology | Chemical Pathology | ||
| Immunoglobulins | Gel/Serum (Brown) | Blood | IgG = 6.0 - 16.0 g/L IgA = 0.8 - 4.0 g/L IgM = 0.5 - 2.0 g/L Age related ranges. Contact laboratory or see report | 1 week (interim report available from lab usually within 1 day) | Barnsley for IgA, IgG and IgM/Rotherham Hospital Immunology for electrophoresis | Chemical Pathology | ||
| Immunoreactive Trypsin | Blood Spot | Blood Spot | With Report | 4 Weeks | Sheffield Children's Hospital | Chemical Pathology | ||
| Inherited Thrombophilia Screen including :- Antithrombin III, Protein C, Protein S, Activated protein C resistance*, Prothrombin gene mutation. *Factor V Leiden genetics will only be carried out if the APCR is abnormal. | Citrate (Green) & EDTA (Red) | Blood | 3 x Green- Should be received in the laboratory within 4hours of venepuncture. 1 x EDTA Individual parts of the Inherited thrombophilia screen can be requested if specified (please see individual sections). NB All requests are audited to ensure that these tests are performed only where appropriate. The criteria for testing are based on BCSHGuideline: Investigation & Management of Heritable Thrombophilia BJH 114:512-528 2001. Requests without clinical details or with inadequate details will not be processed. The samples are saved for 3 months. Please contact Haematology clinicians if the request has been denied and there are other clinical indicators for testing. | Reference ranges are provided with the results. | 4 Weeks | Sheffield Royal Hallamshire Coagulation | Haematology | |
| INR | Citrate (Green) | Blood | Should only be requested if the patient is on warfarin (Vitamin K antagonist therapy). | See BSCH guidelines. | 45 mins | Barnsley Hospital Haematology | Haematology | |
| Insulin | Gel/Serum (Brown) | Blood | 17.8 - 173 pmol/L | 3 Weeks | Sheffield Royal Hallamshire Hospital | Chemical Pathology | ||
| Insulin Antibodies (IgG) | Gel/Serum (Brown) | Blood | Negative = <5 mg/L Equivocal = 5 - 10 mg/L Positive = >10 mg/L | 4 Weeks | Sheffield Protein Reference Unit | Chemical Pathology | ||
| Insulin-like Growth Factor (IGF-1) | Gel/Serum (Brown) | Blood | ug/L Age and sex related ranges. Contact laboratory or see report | 3 Weeks | Sheffield Royal Hallamshire Hospital | Chemical Pathology | ||
| Intermediary Metabolites (Part of hypoglycaemia screen) | Fluoride/Glucose | Blood | Includes: Glucose Lactate Free fatty acids 3-Hydroxybutyrate | Comment issued with report. | 2 Weeks | Sheffield Children's Hospital | Chemical Pathology | |
| Iron | Gel/Serum (Brown) | Blood | Male = 12 - 31 umol/L Female = 9 - 30 umol/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Iron Profile | Gel/Serum (Brown) | Blood | Please see individual tests | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Islet Cell Antibodies | Gel/Serum (Brown) | Blood | Negative (or positive) | 3 Weeks | Sheffield Protein Reference Unit | Chemical Pathology | ||
| JAK-2 (Requested via HODS database) | EDTA (Red) | Bone marrow or peripherial blood. | Only requestable by the Haematologists or after discussion with the Haematology medical team. | See report | Report usually available within 7 days via HODS website. Password access only. | HODS, Sheffield Royal Hallamshire Hospital | Haematology | |
| Lactate | Blood Gas Syringe | Venous Blood Gas For inofmration on interference i nthe lactate assay, please see here | 0.6 - 2.5 mmol/L | As required. (contact the lab) | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Lactate Dehydrogenase (LDH) | Gel/Serum (Brown) | Blood | 120 - 246 U/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Lamotrigine | Gel/Serum (Brown) | Blood | 3.0-14.0 mg/L | 4 Weeks | Chemical Pathology | |||
| Lead | EDTA (Red) | Blood | 1.0 - 35.0 ug/dL In the absence of lead exposure, blood lead levels rarely exceed 10 ug/dL. Values greater than this require investigation to identify and remove the source of the lead | Sheffield Northern General Hospital | Chemical Pathology | |||
| Lipid Profile (Fasting - 12 hours) | Gel/Serum (Brown) | Blood | NB. Consider non-lipid factors in cardio- vascular risk assessment ie. family history of CHD, hypertension, smoking, diabetes mellitus and male gender See NICE CKS for CVD risk assessment: https://cks.nice.org.uk/topics/cvd-risk-assessment-management For information on interferences in our lipid assays, please see here | Total Cholesterol = Desirable <4.0 mmol/L HDL Cholesterol = Desirable >0.90 mmol/L Total:HDL Cholesterol Ratio LDL Cholesterol (calculated) = Desirable <2.0 mmol/L Triglycerides = 0.45 - 1.88 mmol/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | |
| Lithium | Gel/Serum (Brown) | Blood | Therapeutic 0.4 - 1.0 mmol/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Luteinizing hormone (LH) | Gel/Serum (Brown) | Blood | Follicular = 1.9 - 12.5 U/L Mid - Cycle = 8.7 - 76.3 U/L Luteal = 0.5 - 16.9 U/L Post menopausal = 7.9 - 53.8 U/L Adult Male <70 years = 1.5 - 9.3 U/L Adult Male > 70 years 3.1 - 34.6 U/L | <1 day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Magnesium | Gel/Serum (Brown) | Blood | Adult: 0.7 - 1.0 mmol/L Neonate (up to 1 month): 0.6 - 1.0 mmol/L 1 month ? 16 years: 0.7 - 1.0 mmol/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Malarial parasites (including RDT screen, thin and thick films stained at pH7.2) | EDTA (Red) | Blood | Samples must be received into the laboratory within 2 hours of venepuncture. All positive results are referred to Liverpool school of Tropical medicine for confirmatory testing. Positive results are HPA notifiable within 7days of testing | Reported as presence of a species of malaria as an interim report only (with parasitaemia as a % reported for falciparum only) or negative. | 7 Days | Barnsley Hospital Haematology | Haematology | |
| MCH | EDTA (Red) | Blood | Part of FBC. Refer to FBC for request information. Not requestable alone. | 7D = 31.0-39.0 pg 14D = 30.5-37.5 pg 30D = 28.5-35.5 pg 2M = 26.0-33.0 pg 3M = 24.5-30.0 pg 6M = 24.0-29.0 pg 6yr = 24.5-30.0 pg 8yr = 25.2-31.7 pg 10yr = 25.4-31.7 pg 12yr = 26.0-31.7 pg 14yr = 26.9-32.3 pg 16yr = 26.9-32.5 pg >=18yr = 27.0-32.5 pg | Barnsley Hospital Haematology | Haematology | ||
| MCHC | EDTA (Red) | Blood | Part of FBC. Refer to FBC for request information. Not requestable alone. | 3D = 308-365 g/l 7D = 303-368 g/l 14D = 298-373 g/l 2M = 303-368 g/l 3M = 300-365 g/l 4M = 308-365 g/l 2yr = 315-365 g/l >2yr = 316-365 g/l | Barnsley Hospital Haematology | Haematology | ||
| MCV | EDTA (Red) | Blood | Part of FBC. Refer to FBC for request information. Not requestable alone. | 7D = 100-127 fl 14D = 94-126 fl 30D = 90-116 fl 2M = 82-99 fl 3M = 75-95 fl 6M = 70-88 fl 1yr = 71-84 fl 2yr = 73-84 fl 3yr = 73-85 fl 6yr = 75-86 fl 8yr = 75-89 fl 10yr = 76-90 fl 12yr = 77-90 fl 14yr = 78-93 fl 16yr = 79-95 fl 21yr = 82-100 fl | Barnsley Hospital Haematology | Haematology | ||
| Metamyelocytes | EDTA (Red) | Blood | Part of FBC. Not requestable alone. Expressed as an absolute value and percentage of the total white count if seen when examining a blood film | (0.00-0.00) | Barnsley Hospital Haematology | Haematology | ||
| Metanephrines (Plasma) | EDTA (Red) | Plasma |
Please contact lab prior to collecting samples for this test Avoid stress. Samples viable up to 30 min from collection without ice and for 60 mins with ice. This test is subject to interference from a variety of drugs and other agents. Please see the table here for further information | 0 - 510 pmol/L | Freeman Hospital Newcastle | Biochemistry | ||
| Microalbumin - Urine (Albumin:creatinine ratio) | White Top Universal | Urine (random) | Male = <2.5 mg/mmol creatinine Female = <3.5 mg/mmol creatinine | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Monocytes | EDTA (Red) | Blood | Refer to FBC. Not requestable alone | 1D = 0.2-2.3 2D = 0.2-1.8 30D = 0.2-1.5 6M = 0.3-1.2 1yr = 0.2-1.2 3yr = 0.2-1.0 8yr = 0.2-1.2 16yr = 0.2-1.0 >16yr = 0.2-0.8 | Barnsley Hospital Haematology | Haematology | ||
| Myelin Associated Glycoprotein Antibodies | Gel/Serum (Brown) | Blood | With report | 4 Weeks | Sheffield Protein Reference Unit | Chemical Pathology | ||
| Myelocytes | EDTA (Red) | Blood | Refer to FBC. Not requestable alone. Expressed as an absolute value and percentage of the total white count if seen when examining a blood film. | Refer to FBC. | Barnsley Hospital Haematology | Haematology | ||
| Neurone Specific Enolase (NSE) | Gel/Serum (Brown) | Blood | <12.5 ug/L Adult Range Quoted | 4 Weeks | Sheffield Protein Reference Unit | Chemical Pathology | ||
| Neurotensin | Contact Laboratory | BD Gastrin Bottle | See Gut Hormones | 4 Weeks | London, SAS Laboratories | Chemical Pathology | ||
| Neutrophils | EDTA (Red) | Blood | Part of FBC. Not requestable alone. | 1D = 2.8-15.0 2D = 2.8-14.6 8D = 2.8-12.0 14D = 1.8-7.8 30D = 1.8-6.8 2M = 1.0-6.0 3M = 0.8-5.5 6M = 1.0-5.5 1yr = 1.3-7.0 2yr = 1.4-7.5 3yr = 1.5-7.5 4yr = 1.6-7.7 5yr = 1.7-7.8 6yr = 1.7-7.4 8yr = 1.7-7.1 9yr = 1.7-7.2 11yr = 1.7-6.7 >11yr = 1.7-6.6 | Barnsley Hospital Haematology | Haematology | ||
| Oestradiol | Gel/Serum (Brown) | Blood | For information on interference in our oestradiol assay, please see here | Follicular = 72-529 pmol/L Mid-cycle = 235 - 1309 pmol/L Luteal = 205 - 786 pmol/L Post-menopausal = <118 pmol/L Children: F, 2?3 y: <107 pmol/L F, 4?9 y: <160 pmol/L F, 10?11 y: <645 pmol/L F, 12?21 y: <875 pmol/L M, 2?3 y: <189 pmol/L M, 4?9 y: <97 pmol/L M, 10?13 y: <134 pmol/L Adult Male = <146 pmol/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | |
| Oligoclonal bands (CSF) | Plain white top universal form CSF Gel/Serum (Brown) | CSF and Brown Blood Bottle | NB. Blood and CSF required for full interpretation | Narrative report | 3 Weeks | Sheffield Protein Reference Unit | Chemical Pathology | |
| Organic Acids - Urine | White Top Universal | Urine | 5ml. Freeze immediately. | Narrative Report | 4 Weeks | Sheffield Children's Hospital | Chemical Pathology | |
| Osmolality - Serum | Gel/Serum (Brown) | Blood | 275 - 295 mmol/kg | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Osmolality - Urine | White Top Universal | Urine (random) | 50 - 1200 mmol/kg | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| P1NP | Gel/Serum (Brown) | Blood | Please refer to report | 3 Weeks | Sheffield Northern General Hospital | Chemical Pathology | ||
| P3NP (Type III Procollagen Peptide) | Gel/Serum (Brown) | Blood | Please refer to report | 4 Weeks | Manchester, Specialist Assay Laboratory | Chemical Pathology | ||
| Pancreatic Elastase | Blue Top Universal | Faeces | See Elastase/Faecal Elastase | 2 Weeks | Rotherham Hospital Chemical Pathology | Chemical Pathology | ||
| Pancreatic Polypeptide | Contact Laboratory | BD Gastrin Vacutainer | See Gut Hormones | 4 Weeks | London, SAS Laboratories | Chemical Pathology | ||
| Paracetamol | Gel/Serum (Brown) | Blood | Treatment indicated if above treatment line. See BNF For information on interferences in our paracetamol assay, please see here | Significant toxicity likely: >200 mg/L at 4h >50 mg/L at 12h | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | |
| Parathyroid Hormone | EDTA (Red) | Blood | Please send separate EDTA sample for PTH analysis | 1.95-8.49 pmol/L (Please note: new units and range from 30/10/2017) | < 1 Day (Monday -Friday) | Barnsley Hospital Biochemistry | Chemical Pathology | |
| Partial Thromboplastin Time | Citrate (Green) | Blood | Part of the Coagulation Screen. Maximum sample viability: 8hrs. The citrated bottle must be filled up to the green line marked on the bottle label. The ratio of anticoagulant to blood must be 1:9 therefore under or over-filling the bottle may affect the results and samples may not be tested. The ratio of anticoagulant to blood must be 1:9 therefore under or over-filling the bottle may affect the results and samples may not be tested. | For in house testing: 1d = 34.9-47.4 seconds 3d = 31.2-44.0 seconds 1yr = 29.1-35.5 seconds >16yr = >16yr 23.3-33.9 seconds Refer to reference ranges on reports for testing performed as part of any referred tests. | Barnsley Hospital Haematology | Haematology | ||
| Partial Thromboplastin Time Ratio (PTTR) for patients on heparin only. | Citrate (Green) | Blood | Maximum sample viability: 8hrs. The citrated bottle must be filled up to the green line marked on the bottle label. The ratio of anticoagulant to blood must be 1:9 therefore under or over-filling the bottle may affect the results and samples may not be tested. The ratio of anticoagulant to blood must be 1:9 therefore under or over-filling the bottle may affect the results and samples may not be tested. | 1.5-2.5 | Barnsley Hospital Haematology | Haematology | ||
| Phenobarbitone | Gel/Serum (Brown) | Blood | For ? Poisoning telephone lab on 2733 to arrange for urgent courier | Therapeutic 10 - 40 mg/L | <1 Day | Sheffield Northen General Hospital | Chemical Pathology | |
| Phenytoin | Gel/Serum (Brown) | Blood | Therapeutic 5 - 20 mg/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Phosphate - Serum | Gel/Serum (Brown) | Blood | 0.80 - 1.50 mmo/L Adult range stated. Please contact lab for age related ranges. | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Phosphate - Urine | 24 Hour Acid HCL Urine Container | Urine | 15 - 50 mmol/24 hours | <1 Day | Chemical Pathology | |||
| PIVKA II | Citrate (Green) | Blood | Discuss with Haematology Consultants for testing approval. The citrated bottle must be filled up to the green line marked on the bottle label. The ratio of anticoagulant to blood must be 1:9 therefore under or over-filling the bottle may affect the results and samples may not be tested. Send immediately to the labs for pre analytical processing. | See referral report | 2 Weeks | Sheffield, Royal Hallamshire Hospital, Coagulation | Haematology | |
| Placental Alkaline Phosphatase (PLAP) | Gel/Serum (Brown) | Blood | Non-smoker = <500 mU/L Smoker = <1500 mU/L | 4 Weeks | Sheffield Protein Reference Unit | Chemical Pathology | ||
| Plasma Viscosity | EDTA (Red) | Blood | Only requestable via Haematology Consultants. | See referral report | 2 Weeks | Sheffield, Royal Hallamshire Hospital, Haematology | Haematology | |
| Platelet Clumping | Citrate (Green) | Blood | Occasionally a patient?s platelets react to the EDTA anticoagulant used. The laboratory may request an additional sample to verify this and report an accurate platelet count. If a green (citrated) sample is requested this must be sent with an EDTA for FBC testing. | Reference ranges same as platelets. | As per FBC | Barnsley Hospital Haematology | Haematology | |
| Platelets | EDTA (Red) | Blood | Not requestable alone. Refer to the FBC section. For platelet clumping requests, see platelet clumping. | 3D = 125-450 x 10^9/L 7D = 150-450 x 10^9/L 30D = 150-475 x 10^9/L 2M = 180-550 x 10^9/L 3M = 210-650 x 10^9/L 6M = 210-600 x 10^9/L 1yr = 190-530 x 10^9/L 3yr = 190-500 x 10^9/L 6yr = 190-490 x 10^9/L 8yr = 190-440 x 10^9/L 10yr = 185-425 x 10^9/L 12yr = 180-405 x 10^9/L 14yr = 180-400 x 10^9/L 19yr = 170-400 x 10^9/L >21yr = 150-450 x 10^9/L | As per FBC | Barnsley Hospital Haematology | Haematology | |
| PNH screen (Peroxysmal nocturnal haemolysis screen) | EDTA (Red) | Blood | Only requestable via Haematology Consultants. | See HODS Report | 4 Weeks | HODS, Sheffield Royal Hallamshire Hospital | Haematology | |
| Porphyrins/Porphyria - full screen | 2xEDTA (Red) White top Universal container (Urine) Blue Top Stool Container PROTECT ALL SAMPLES FROM LIGHT | EDTA x2 , Fresh random urine sample and small portion of faeces required. | *Protect all samples from light | Reference ranges and comment with report. | 3 Weeks | Cardiff, Department of Medical Biochemistry | Chemical Pathology | |
| Post Vasectomy Semen Analysis | Contact Laboratory | Semen | The RCOG (Royal College of Obstetricians) and FPA (Family Planning Association) state that at least two azoospermic, consecutive samples, two to four weeks apart, must be obtained before contraceptive precautions can be dispensed with. NOTE: the laboratory is not accredited for this assay. Initial assessment should be undertaken 16 weeks post vasectomy and after the patient has produced at least 24 ejaculates. A second sample should be submitted 2-4 weeks later. Semen should be collected after an abstinence period of no less than 48 hours and no more than seven days, and maintained at body temperature before delivery to the laboratory. The laboratory must receive the sample within 1 hour of production. Semen samples will only be accepted for testing when received in a pre-weighed, sterile pot provided by the laboratory and with a fully completed request form. Missing information or patient demographic detail may result in the specimen being rejected. Please refer to the information on the request form. Results may also be affected by surgery, previous infection, recent illness or high temperature, prescribed or recreational drugs. Please contact the laboratory if in doubt. | Negative | 1 Week | Haematology | ||
| Potassium (Serum) | Gel/Serum (Brown) | Blood | Adult: 3.5?5.3 mmol/L Neonate (up to 1 month): 3.4 - 6.0 mmol/L Infant (1 month to 1 year): 3.5 - 5.7 mmol/L 1 year to 16 years: 3.5 ? 5.0 mmol/L Adult 3.5 - 5.3 mmol/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Potassium (Urine) | White Top Universal (for random urine) or 24 Hour Plain Urine Container (for 24 hour urine) | Urine | Very variable - dependant on dietary intake and should be interpreted with the serum U&Es | 25 - 125 mmol/24hr | <1 Day | Chemical Pathology | ||
| Pregnancy Test - Urine | White Top Universal | Urine (Early morning collection) | Negative Positive (3-7 days after first missed menstrual peroid) | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Progesterone | Gel/Serum (Brown) | Blood | For information on interferences in our progesterone assay, please see here | Male: 0.89?3.88 nmol/L Normally ovulating females: Follicular phase: 0 - 4.45 nmol/L) Luteal: 10.6 - 81.3 nmol/L Mid-luteal: 14.1 - 89.1 nmol/L Female, postmenopausal: 0 - 2.3 nmol/L Female, pregnant: 1st trimester: 35.7 ? 286.2 nmol/L 2nd trimester: 81.3?284.3 nmol/L 3rd trimester: 153.9 ? 1343.6 nmol/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | |
| Proinsulin | Gel/Serum (Brown) | Blood | On ice and separated promptly. | With report | 4 Weeks | Chemical Pathology | ||
| Prolactin | Gel/Serum (Brown) | Blood | , nonpregnant: 59?619 mIU/L M: 45?375 mIU/L F, pregnant: 206?4420 mIU/L F, postmenopausal: 38?430 mIU/L Children: F, 2?3 y: 65.7?332.8 mIU/L F, 4?9 y: 66.6?334.1 mIU/L F, 10?12 y: 75.0?386.7 mIU/L F, 13?21 y: 89.9?489.7 mIU/L M, 2?3 y: 76.3?606.3 mIU/L M, 4?9 y: 95.4?382.2 mIU/L M, 10?16 y: 67.8?284.9 mIU/L M, 17?21 y: 115.1?326.7 mIU/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Promyelocytes | EDTA (Red) | Blood | Part of FBC. Not requestable alone. Refer to the FBC section. Expressed as an absolute value and percentage of the total white count if seen when examining a blood film. | (0.00-0.00) | Barnsley Hospital Haematology | Haematology | ||
| Prostate Specific Antigen (PSA) | Gel/Serum (Brown) | Blood | PSA is raised in Ca. prostate and in 25 - 30% of patients with BPH. PSA samples should not be taken from men who have: - an active UTI - ejaculated in the previous 48 hours - exercised vigorously in the previous 48 hours - had a prostate biopsy in the previous 6 weeks. - had a DRE in the previous week. N.B PSA is not tumour specific and is elevated in benign prostatic hypertrophy, prostatis etc. | Please refer to NICE guidelines | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | |
| Protein - Urine | White Top Universal | Urine | EMU is now preferred or 24 hour. Samples containing the antibiotics amikacin, gentamicin, kanamycin, and tobramycin should be avoided since these substances falsely increase the urine protein results in our current method | Protein:creatinine ratio (PCR) = <50 mg/mmol creatinine Albumin:creatinine ratio (ACR) = <30mg/mmol creatinine 24hr urine protein = <0.15 g/24 hours | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | |
| Protein (Total) - Serum | Gel/Serum (Brown) | Blood | 57 - 76 g/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Protein C (PC) | Citrate (Green) | Blood | Part of the Inherited thrombophilia screen. Can be requested as an individual test if written in the clinical details. 1 x green sample if requesting alone. Otherwise, refer to Thrombophilia screen. Discuss with Haematology Consultants for testing approval. The citrated bottle must be filled up to the green line marked on the bottle label. The ratio of anticoagulant to blood must be 1:9 therefore under or over-filling the bottle may affect the results and samples may not be tested. Send immediately to the labs for pre analytical processing. | Report from the referral laboratory. | Refer to the Inherited thrombophilia screen | Sheffield Royal Hallamshire Hospital, Coagulation | Haematology | |
| Protein Electrophoresis | Gel/Serum (Brown) | Blood | Narrative Report | 1 Week | Rotherham Hospital Immunology | Chemical Pathology | ||
| Protein S (PS) | Citrate (Green) | Blood | Part of the Inherited thrombophilia screen. Can be requested as an individual test if written in the clinical details. Test authorised by Haematology consultants only if the request is done in accordance with the guidelines. Appropriate clinical information must be provided or the request may be rejected. The citrated bottle must be filled up to the green line marked on the bottle label. The ratio of anticoagulant to blood must be 1:9 therefore under or over-filling the bottle may affect the results and samples may not be tested. Send immediately to the labs for pre analytical processing. | Report from the referral laboratory. | Refer to the Inherited thrombophilia screen | Sheffield Royal Hallamshire Hospital, Coagulation | Haematology | |
| Prothrombin 20210A allele genetics (PGM) | EDTA (Red) | Blood | Part of the Inherited thrombophilia screen. Can be requested as an individual test. Discuss with Haematology Consultants for testing approval. Send immediately to the labs for pre analytical processing. | Report from the referral laboratory. | Refer to the inherited thrombophilia screen | Sheffield Royal Hallamshire Hospital, Coagulation | Haematology | |
| Prothrombin Time | Citrate (Green) | Blood | The citrated bottle must be filled up to the green line marked on the bottle label. The ratio of anticoagulant to blood must be 1:9 therefore under or over-filling the bottle may affect the results and samples may not be tested. Refer to the Coagulation Screen for full test requirements. | For in house testing: 1 Day = 14.4-16.4 Seconds 3 Day = 13.5-16.4 Seconds <1 Month = 10.0 -15.0 Seconds >=1 Month = 10.0-14.0 Seconds 1 Year = 11.5-15.3 Seconds >16 Years = 12.4-17.3 seconds | Barnsley Hospital Haematology | Haematology | ||
| Pseudocholinesterase (for scoline apnoea) | EDTA (Red) | Blood | With report Details of anaesthetic sensitivity required | 4 Weeks | Bristol Cholinesterase Investigation Unit | Chemical Pathology | ||
| Quinine | Gel/Serum (Brown) | Blood | Therapeutic = 3 - 7 mg/L | 4 Weeks | Sheffield Royal Hallamshire Hospital | Chemical Pathology | ||
| RBC | EDTA (Red) | Blood | Part of the FBC. | 3D = 3.85-6.50 7D = 3.85-6.40 14D = 3.53-6.20 30D = 3.10-5.20 2M = 2.90-4.60 3M = 3.10-4.65 4M = 3.20-5.00 6M = 3.40-5.10 1yr = 3.70-5.10 6yr = 3.90-5.10 8yr = 4.00-5.10 12yr = 4.15-5.20 Male 15yr = 4.38-5.60 Male 18-20yr = 4.42-5.60 Male >=21yr = 4.33-5.60 Female 15yr = 4.04-5.10 Female 18-20yr = 4.00-5.10 Female >=21yr = 3.85-4.90 | Barnsley Hospital Haematology | Haematology | ||
| RBC | EDTA (Red) | Blood | Part of the FBC. | 12.2-15.4 | Barnsley Hospital Haematology | Haematology | ||
| Red cell membrane testing | EDTA (Red) | Blood | Refer to EMA screen for full requirements. | Haematology | ||||
| Renal Calculi | White Top Universal | Calculi | Narrative Report | 4 Weeks | City Hospital Birmingham | Chemical Pathology | ||
| Renin (PRA - plasma renin activity) | Lithium Heparin (Orange) | Blood | After overnight recumbency = 1.1 - 2.7 nmol/L/h After 30 min upright = 2.8 - 4.5 nmol/L/h When taken at randomduring the day = 0.5 - 3.5 nmol/L h | 2 Weeks | Leeds SAS Centre for Steroid Hormones | Chemical Pathology | ||
| Reticulocytes | EDTA (Red) | Blood | <3D = 110-450 x 10^9/L 3D = 50-150 x 10^9/L >=4D = 20-100 x 10^9/L | Haematology | ||||
| Rheumatoid Factor | Gel/Serum (Brown) | Blood | 0 - 13 U/mL | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Salicylate | Gel/Serum (Brown) | Blood | For information on interferences in our salicylate assay, please see here | Therapeutic = <250 mg/L Toxicity likely if = >500 mg/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | |
| Selenium | Gel/Serum (Brown) | Blood | 0.61 - 1.24 umol/L | 4 Weeks | Sheffield, Northern General Hospital | Chemical Pathology | ||
| Sex Hormone Binding Globulin (SHBG) | Gel/Serum (Brown) | Blood | Adult Male <50 years = 14.6 -94.6 nmol/L Adult Male >50 years = 21.6-113.3 nmol/L Female pre-menopausal = 10.8- >180 nmol/L Female post-menopausal = 23.2 -159.1 nmol/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Sezary cells Includes: FBC, Blood film & cell markers | EDTA (Red) | Blood | 2 x EDTA samples required. Samples should not be taken on Fridays. | Negative | 4 Weeks | Sheffield Royal Hallamshire Hospital, Cell Marker Laboratory | Haematology | |
| Sickle screen (includes quantitative assay initially followed by Ferritin & HPLC) | EDTA (Red) and Gel/Serum (Brown) | 1 x EDTA Blood 1 x Brown Top Blood | For urgent pre-op/sickle crisis only. Urgent requests must be phoned to the laboratory. All requests are followed up with HPLC. | Negative | Urgent HbS screen performed immediately (TAT 1hr). TAT 72hrs for HPLC. | Rotherham Hospital Haematology | Haematology | |
| Sodium (Serum) | Gel/Serum (Brown) | Blood | 133 - 146 mmol/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Sodium (Urine) | White Top Universal (for random urine) or 24 Hour Plain Urine Container (for 24 hour urine) | Urine | Very variable - dependent on dietary intake and should be interpreted with the serum U&Es | 40 - 220 mmol/24hr | <1 Day | Chemical Pathology | ||
| T315 mutation | EDTA (Red) | Blood or Bone Marrow | Only requested by Consultant Haematologists | Refer to HODS database. | 8-12 Weeks | Haematology | ||
| Tacrolimus | EDTA (Red) | Blood | EDTA Trough Level Please specify transplant type and Unit | Dependent on clinical indication. | 4 Weeks | Sheffield Northern General Hospital, or to appropriate Transplant Unit (where possible) | Chemical Pathology | |
| T-cell gene rearrangement | EDTA (Red) | Blood | Sample viability: 24hrs. Samples should not be taken on Fridays. Send to the Blood Sciences laboratory immediately so samples can be referred. Samples should be booked into the HODS database by the requestor. If in doubt, please contact the Blood Sciences laboratory | Report available on the HODS database. Password access only. | 8 Weeks | HODS, Sheffield Royal Hallamshire Hospital | Haematology | |
| T-cell subsets | EDTA (Red) | Blood | Samples should not be taken on Fridays. Send to the Blood Sciences laboratory immediately so samples can be referred. Samples should be booked into the HODS database by the requestor. If in doubt, please contact the Blood Sciences laboratory | Report available via HODS database or as a hardcopy. | 4 Weeks | HODS, Sheffield Royal Hallamshire Hospital | Haematology | |
| Temazepam | Gel/Serum (Brown) | Blood | 400 - 850 ug/L | 4 Weeks | Sheffield, Royal Hallamshire Hospital | Chemical Pathology | ||
| Testing for Direct oral anticoagulants (DOAC), including Dabigatran, apixaban, rivaroxaban | Citrate (Green) | Blood | NOTE: This is not an assay for monitoring therapy. Type of drug, Date and time of last dose required. | Refer to external report. | 2 Weeks | Sheffield Royal Hallamshire Hospital, Coagulation | Haematology | |
| Testosterone | Gel/Serum (Brown) | Blood | For information on interferences in our testosterone assay, please see here | Adult Male = 9.3 -32.2 nmol/L Female pre-menopausal = 0.4-2.1nmol/L Female post-menopausal = <0.2-1.7 noml/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | |
| Theophylline | Gel/Serum (Brown) | Blood | Therapeutic = 10 - 20 mg/L | <1 Day | Barnsley Hospital Biochemistry | Chemical Pathology | ||
| Thiamine (Vitamin B1) | EDTA (Red) | Blood | Protect from light. | 66.5-200 nmol/L | 2 weeks | Rotherham Hospital Chemical Pathology | Chemical Pathology | |
| Thiopurine Methyltransferase | EDTA (Red) | Blood | Normal = 26 - 50 pmol/h/mgHb Deficiency = <10 pmol/h/mgHb Carrier = 10 - 25 pmol/h/mgHb With comment on report. | 4 Weeks | City Hospital Birmingham, Trace Elements laboratory | Chemical Pathology | ||